Immobilization of bioluminescent Escherichia coli cells using natural and artificial fibers treated with polyethyleneimine |
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Authors: | Yi-Fang Chu Chia-Hua Hsu Pavan K. Soma Y. Martin Lo |
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Affiliation: | 1. Department of Animal and Food Sciences, University of Delaware, 044 Townsend Hall, 531 S. College Ave., Newark, DE 19717, USA;2. Department of Nutrition and Food Science, University of Maryland, 0112 Skinner Building, College Park, MD 20742, USA |
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Abstract: | Biosensors based on whole-cell bioluminescence have the potential to become a cost-effective alternative to conventional detection methods upon validation of target selectivity and sensitivity. However, quantitative analysis of bioluminescence is greatly hindered due to lack of control over the total number of cells in a suspending culture. In this study, the effect of surface properties of genetically engineered luminous E. coli cells and fibrous matrices on the immobilization capacity and effectiveness under various environmental conditions were characterized. Four different fibers, including cotton, polyester, viscose rayon, and silk, were investigated. Although cell adhesion was observed on untreated viscose and cotton fibers, viscose fiber pretreated with 0.667% polyethyleneimine (PEI) was found capable of immobilizing the most viable E. coli DPD2234 cells, followed by viscose treated with 0.33% and 1% PEI. The cells immobilized on PEI-treated viscose remained viable and yielded 20% or more bioluminescence signals immediately upon contact with the inducer up to 72 h without feeding nutrients to the cells, suggesting that viscose treated with 0.667% PEI could provide a stable immobilization mechanism for bioluminescent E. coli cells with long sensing period, quick response time, and good signal reproducibility. |
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Keywords: | Bioluminescence Cell immobilization Crosslinking agent Lux Fiber |
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