3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp. SK007 |
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Authors: | Wanmeng Mu Fengli Liu Jianghua JiaChao Chen Tao ZhangBo Jiang |
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Affiliation: | State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China |
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Abstract: | 3-Phenyllactic acid (PLA), which is produced by some strains of lactic acid bacteria (LAB), is a known antimicrobial agent with a broad spectrum. Batch and fed-batch fermentation by the strain Lactobacillus sp. SK007 for PLA production have been reported. With batch fermentation without pH-control, PLA production yield was 2.42 g L−1. When fed-batch fermentation by Lactobacillus sp. SK007 was conducted in 3 L initial volume with pH-control at 6.0 and intermittent feeding, which was developed after fermentation for 12 h and every 2 h with 120 mL 100 g L−1 PPA phenylpyruvic acid (PPA) and 50 mL 500 g L−1 glucose each time, PLA production yield reached 17.38 g L−1. The final conversion ratio of PPA to PLA was 51.1%, and the PLA production rate was 0.241 g L−1 h−1. This indicated that PPA was the ideal substrate for PLA fermentation production, and fed-batch fermentation with intermittent PPA feeding and pH-control was an effective approach to improve PLA production yield. |
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Keywords: | 3-Phenyllactic acid Lactobacillus sp. SK007 Fed-batch fermentation Intermittent feeding pH-control |
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