DCCD inhibits proton translocation and electron flow at the second site of the mitochondrial respiratory chain |
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Authors: | G Lenaz M D Esposti G Parenti Castelli |
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Affiliation: | 1. Istituto Botanico, University of Bologna, 40126 Bologna, Italy;2. Istituto di Chimica Biologica, University of Bologna, 40126, Bologna, Italy |
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Abstract: | DCCD inhibits formation of a succinate-driven transmembrane pH gradient in submitochondrial particles, as shown by inhibition of fluorescence quenching of 9-aminoacridine, without concomitant inhibition of succinate oxidation. On the other hand ubiquinol-cytochrome c reductase activity is inhibited by DCCD. Half-inhibition of both fluorescence quenching and ubiquinol-cytochrome c reductase occur at 35 μM DCCD. The results suggest that DCCD inhibits proton pumping activity coupled to electron flow through the bc1 complex. |
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