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双孢蘑菇基质降解能力退化的差异蛋白质组学分析
引用本文:陈美元,王泽生,廖剑华,李洪荣,蔡志欣,谢宝贵. 双孢蘑菇基质降解能力退化的差异蛋白质组学分析[J]. 菌物学报, 2011, 30(3): 508-513
作者姓名:陈美元  王泽生  廖剑华  李洪荣  蔡志欣  谢宝贵
作者单位:1. 福建省农业科学院食用菌研究所,福州,350014;福建农林大学茵物研究中心,福州,350002
2. 福建省农业科学院食用菌研究所,福州,350014
3. 福建农林大学茵物研究中心,福州,350002
基金项目:国家自然科学基金(No. 30871769),福建省重大专题(No. 2008SZ0002)
摘    要:对双孢蘑菇CGMCC No. 0214及其基质降解能力退化菌株0214-3、0214-5进行了蛋白质双向电泳(2-DE或2D-PAGE)分析,发现了2个明显的、可重复的差异蛋白质,在退化菌株中分别为上调和下调表达,且2个退化菌株表现一致。根据质谱(MALDI-TOF/MS)分析和数据库检索结果,2个差异蛋白质初步鉴定为肌动蛋白和NADH脱氢酶铁硫蛋白3。

关 键 词:食用菌  蛋白质组  双向电泳  差异蛋白质  质谱分析

Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus
CHEN Mei-Yuan,WANG Ze-Sheng,LIAO Jian-Hu,LI Hong-Rong,CAI Zhi-Xin and XIE Bao-Gui. Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus[J]. Mycosystema, 2011, 30(3): 508-513
Authors:CHEN Mei-Yuan  WANG Ze-Sheng  LIAO Jian-Hu  LI Hong-Rong  CAI Zhi-Xin  XIE Bao-Gui
Affiliation:Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, ChinaMycological Research Center of Fujian Agriculture and Forestry University, Fuzhou 350002, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China;Mycological Research Center of Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract:The technique of two-dimensional electrophoresis (2-DE or 2D-PAGE) was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No. 0214 and its substrate-decomposing ability degenerated strains 0214-3, 0214-5. As a result, two proteins were found to be up- and down-expressed, respectively, with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively. The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A. bisporus.
Keywords:edible fungus   proteome   two-dimensional electrophoresis   differential protein   MALDI-TOF/MS analysis
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