Affiliation: | 1.Russian Cardiology Research and Production Complex,Ministry of Health of the Russian Federation,Moscow,Russia;2.Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center,Russian Academy of Sciences,Moscow,Russia |
Abstract: | The interaction of superoxide radical anion (O2 ??) with active dicarbonyls (methylglyoxal, glyoxal, and malonic dialdehyde) was studied. It was demonstrated that glyoxal and methylglyoxal inhibited superoxide-dependent accumulation of formazan; however, malonic dialdehyde stimulated this process. The formation of O2 ?? in these experiments occurred during the decomposition of the SOTS-1 azo initiator. On the other hand, all of the studied dicarbonyls in this system of O2 ?? generation competed for superoxide with the TIR ON spin trap. These compounds also inhibited luminal-dependent chemiluminescence during the AIBN azo initiator-induced peroxidation of liposomes from the egg phosphatidylcholine. A mechanism for the antiradical and antioxidant effects of the studied dicarbonyls, assuming the production of free radical intermediates in their reactions with O2 ?? or its protonated form, is proposed. |