Involvement of Rhizobium leguminosarum nodulation genes in gene expression in pea root hairs |
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Authors: | Ton Gloudemans T. V. Bhuvaneswari Marja Moerman Ton van Brussel Albert van Kammen Ton Bisseling |
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Affiliation: | (1) Department of Molecular Biology, Agricultural University, Dreyenlaan 3, NL-6703 HA Wageningen, Netherlands;(2) Battelle Research Laboratorium, 7 Rue de Drize, CH-1227 Carouge Geneva, Switzerland;(3) Present address: Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL-2311 VJ Leiden, Netherlands;(4) Laboratory for Physiological Chemistry, State University Utrecht, Vondellaan 24A, NL-3521 GG Utrecht, Netherlands |
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Abstract: | The mRNA population in pea root hairs was characterized by means of in vitro translation of total root hair RNA followed by 2-dimensional gel electrophoresis of the translation products. Root hairs contain several mRNAs not detectable in total RNA preparations from roots. Most of these root hair-specific mRNAs occur in elongating root hairs at higher levels than in mature root hairs. The expression of some genes in pea root hairs is typically affected by inoculation with Rhizobium leguminosarum. One gene, encoding RH-42, is specifically induced while the expression of another gene, encoding RH-44, is markedly enhanced. Using R. leguminosarum mutants it was shown that the nodC gene is required for the induction and enhancement of expression of the RH-42 and RH-44 genes, respectively, while the Rhizobium chromosomal gene pss1, involved in exopolysaccharide synthesis, is not essential. After induction of the nod genes with apigenin the bacteria excrete into the culture medium a factor that causes root hair deformation. This deformation factor stimulates the expression of the RH-44 gene but does not induce the expression of the gene encoding RH-42. |
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Keywords: | deformation factor gene expression pea Rhizobium leguminosarum root hairs |
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