A set of polymorphic DNA microsatellites useful in swamp and river buffalo (Bubalus bubalis) |
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| Authors: | S S Moore D Evans K Byrne J S F Barker S G Tan D Vankan D J S Hetzel |
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| Institution: | CSIRO, Division of Tropical Animal Production, Molecular Animal Genetics Centre, University of Queensland, Brisbane 4068, Australia;Department of Animal Sciences, University of New England, Armidale, New South Wales 2351, Australia;Department of Biology, Universiti Pertanian Malaysia, Serdang, Selangor, Malaysia;Cattle Blood Typing Laboratory, Department of Veterinary Pathology, University of Queensland, Brisbane, Australia |
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| Abstract: | DNA microsatellites have found widespread application in gene mapping, pedigree determination and population genetics. In closely related species such as bovids, heterologous polymerase chain reaction (PCR) primers may in some cases be used, bypassing the need to isolate and characterize microsatellite-containing sequences and design PCR primers. We report on the ability of a set of eighty bovine derived DNA microsatellite primers to amplify sequences in the two types (swamp and river) of water buffalo ( Bubalus bubalis ). Number of alleles and per cent heterozygosities in a large number of animals were determined on a subset of microsatellite loci selected on the robustness of the primers. These loci will form the basis of a set of polymorphic DNA markers for use in water buffalo. |
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| Keywords: | bovine Bubalus bubalis DNA microsatellites heterologous primers PCR water buffalo |
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