Effect of medium on the bacteriocin production byKlebsiella pneumoniae |
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Authors: | S Chhibber D V Vadehra |
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Institution: | (1) Department of Microbiology, Panjab University, 160 014 Chandigarh, India;(2) Present address: Westreco Inc., Boardman Road, 06776 New Milford, Connecticut, USA |
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Abstract: | The influence of growth media and media constituents on bacteriocin production byKlebsiella pneumoniae was studied. Among the standard laboratory media used trypticase soy broth (TSB) showed the maximum production and poor yields
resulted from growth in peptone water and nutrient broth. A number of peptones differed in their bacteriocin production. Best
yields were observed in tryptone and proteose peptone water. Addition of 1 % yeast extract to TSB further stimulated bacteriocin
production. However, activity was low when glucose, glycine, sodium mercaptoacetate or bile salt mixture were added to the
medium. Supression of synthesis by certain agents as well as inhibition of formed bacteriocin by the others appears to affect
the bacteriocin yield. No proteinase activity was detected during the entire incubation period. |
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