The effect of 2,4-dichlorophenoxyacetic acid and (2-chloroethyl)-trimethylammonium chloride on chlorophyll synthesis in barley leaves |
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Authors: | P R Shewry N J Pinfield A K Stobart |
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Institution: | (1) Department of Botany, The University, Bristol, U.K. |
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Abstract: | Summary 2.4-dichlorophenoxyacetic acid (2.4-D) and (2-chloroethyl)-trimethylammonium chloride (CCC) inhibit chlorophyll synthesis and protochlorophyllide 652 regeneration in 6–8 day old barley leaves whilst having little effect on the rates of protochlorophyll 632 synthesis from exogenous -aminelevulinic acid (ALA) and ALA-dehydratase activity. Longer pretreatments with 2.4-D and CCC show it is only after 50 to 60 hr that the rates of P632 production from exogenous ALA and ALA-dehydratase activity are affected. Similar response times were obtained for chloramphenicol (CAP). The results indicate that 2.4-D and CCC may act by directly inhibiting specific plastid-protein synthesis similar to CAP. Hence it seems that it is only those proteins (enzymes) having a rapid turnover that are affected first i.e. the enzymes necessary for ALA synthesis in the plastid.Abbreviations used ALA
-aminolevulinic acid
- CAP
chloramphenicol
- CCC
(2-chloroethyl)-trimethylammonium chloride
- 2.4-D
2-4-dichlorophenoxyacetic acid
- P652
prodochlorophyllide with maximum in-vivo absorption at 652 nm
- P684
chlorophyllide absorbing at 684 nm
- P670
chlorophyllide absorbing at 670 nm
- P632
pigment absorbing at 632 nm synthesised from exogenous ALA
- PBG
Porphobilinogen
P. R. Shewry is in receipt of a Science Research Council Studentship award. |
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