Biomolecular structure refinement based on adaptive restraints using local-elevation simulation |
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Authors: | Markus Christen Bettina Keller Wilfred F van Gunsteren |
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Institution: | (1) Laboratory of Physical Chemistry, Swiss Federal Institute of Technology Zürich, ETH-Zürich, 8093 Zürich, Switzerland |
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Abstract: | Introducing experimental values as restraints into molecular dynamics (MD) simulation to bias the values of particular molecular
properties, such as nuclear Overhauser effect intensities or distances, dipolar couplings, 3
J-coupling constants, chemical shifts or crystallographic structure factors, towards experimental values is a widely used structure
refinement method. Because multiple torsion angle values ϕ correspond to the same 3
J-coupling constant and high-energy barriers are separating those, restraining 3
J-coupling constants remains difficult. A method to adaptively enforce restraints using a local elevation (LE) potential energy
function is presented and applied to 3
J-coupling constant restraining in an MD simulation of hen egg-white lysozyme (HEWL). The method succesfully enhances sampling
of the restrained torsion angles until the 37 experimental 3
J-coupling constant values are reached, thereby also improving the agreement with the 1,630 experimental NOE atom–atom distance
upper bounds. Afterwards the torsional angles ϕ are kept restrained by the built-up local-elevation potential energies. |
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Keywords: | Molecular dynamics simulation 3 J-coupling constant Local-elevation search Restrained simulation Hen egg white lysozyme Gromos" target="_blank">Gromos Force field Structure determination |
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