Effect of aging on the response of biochemical markers in the rabbit subjected to short-term partial bladder obstruction |
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Authors: | Ahmet Guven Wei-Yu Lin Robert E Leggett Barry A Kogan Robert M Levin Anita Mannikarottu |
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Institution: | (1) Department of Pediatric Surgery, Gulhane Military Medical Faculty, Ankara, Turkey;(2) Albany College of Pharmacy, 106 New Scotland Ave, Albany, NY 12208, USA;(3) Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chia-Yi, Taiwan, ROC;(4) Albany Medical College, Albany, NY, USA;(5) Stratton VA Medical Center, Albany, NY, USA |
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Abstract: | Purpose Partial bladder outlet obstruction (PBOO) results in marked biochemical alterations in the bladder. In this study, we focused
on comparison of thapsigargin sensitive sarco/endoplasmic reticulum Ca2+ ATPase activity (SERCA) and Citrate Synthase after short term PBOO in young versus old rabbits. Materials and methods A total of 20 young and 20 mature male rabbits were divided into 4 sub-groups of 5 rabbits each (4 obstructed and 1 sham-control
rabbit). The rabbits in the groups were evaluated after 1, 3, 7, and 14 days of obstruction, respectively. The activities
of SERCA and citrate synthase were examined as markers for sarcoplasmic reticular calcium storage and release and mitochondrial
function, respectively. Results The SERCA activity of bladder body smooth muscle in the young animals increased at 7 and 14 days. For the old rabbits, the
SERCA activity decreased significantly by 1 day and remained this level throughout the course of obstruction, and was significantly
lower than young at all time periods. The citrate synthase activity in the young animals decreased over the 1–7 days, and
then returned toward control level by 14 days following obstruction. In the old animals, citrate synthase activity of bladder
body smooth muscle progressively decreased over the course of the study, and was significantly lower in the old than the young
animals after 14 days obstructed. Conclusion The urinary bladders of the young rabbits have a considerable greater ability to adapt to PBOO than do those of the old rabbits.
The deterioration of mitochondrial and SR function may be important mechanisms underlying geriatric voiding dysfunction. |
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Keywords: | Bladder Citrate synthase SERCA Obstruction Mitochondria Sarcoplasmic reticulum |
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