Expression ofHistocompatibility-2 antigens on cultured cell lines derived from mouse blastocysts |
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Authors: | Suzanne Ostrand-Rosenberg Craig Hammerberg Michael Edidin Michael I Sherman |
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Institution: | (1) Biology Department, The Johns Hopkins University, 21218 Baltimore, Maryland;(2) Roche Institute of Molecular Biology, 07110 Nutley, New Jersey |
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Abstract: | Four cell lines derived from four-day-old SWR/J ×SJL/J mouse blastocysts have been assayed for their expression of H-2 specificities with pauci- and monospecific H-2 typing sera. Direct microcytotoxicity and indirect absorption studies reveal many deviations from expected expression of particular H-2 specificities based on the cell lines' genotypes and onH-2 typing of adult F1 lymphocytes. No pattern of selective expression of public or private specificities ofD-end orK- end specificities or of inclusion groups was noted. At least one public or private specificity of eachD
q
,K
q
,D
s
, andK
s
region is present, indicating that part of each product is expressed. The partial expression of H-2 specificities is discussed structurally, in terms of how incomplete H-2 molecules may be present on the cell surface, and developmentally, in terms of how the variant H-2 specificities may be involved in cell positioning during ontogenesis. |
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