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Isolation of a histone-specific proteinase from brain cell nuclei of rats] |
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| Authors: | A F Protas P P Chaialo |
| Abstract: | A method for isolating histone proteinase of rat brain chromatin is described, including ammonium sulphate fractionation gel filtration on sefacryl 5200, ion exchange chromatography on mono S and affinity chromatography with benzamidine sefarose. The enzyme molecular weight equals 25 kDa. It is purified 15621 times in comparison with initial nuclear extract. |
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