1株产多糖芽胞杆菌的分子鉴定和诱变选育

以中国农业科学院北京畜牧兽医研究所鸡舍附近土壤为材料,采用稀释平板法对其中的菌株进行分离与纯化培养,并对其进行形态学鉴定和粗多糖提取量的检测,同时对分离得到的菌株进行紫外线和亚硝基胍的诱变。从土壤中分离得到1株产多糖的芽胞杆菌(编号P-30),结合形态学鉴定、16S rRNA序列分析和系统发育树分析结果,确定该菌株为短短芽胞杆菌(Bacillus brevis)。其16S rRNA GenBank登录号为HM185814。经过诱变选育后,获得3株(N-05、N-11、U-01)多糖产量为P-30的1.44、1.44和1.29倍的诱变菌株,具有良好的开发前景。

Molecular Characterization and Mutation Breeding of a Polysaccharide Producing Bacillus Strain

With soil samples collected near the chicken house in Beijing Institute of Husbandry and Veterinary of Chinese Academy of Agriculture Sciences as the materials,the strains were isolated from the samples,cultured and purified by diluted plate method.And the morphological identification and the polysaccharide content of isolated strains were determined.The isolated strain was mutated by UV and nitrosogudnidine（NTG）.A polysaccharide producing Bacillus P-30 strain was isolated and identified as Bacillus brevis by analyses of its morphological,16S rRNA sequence（Gene Bank Accession Number： HM185814） and phylogenetic tree analysis.Three mutation strains（N-05,N-11,and U-01） were obtained.They produced polysaccharide 1.44,1.44 and 1.29 times the yield of the P-30 strain respectively.The polysaccharide producing strains obtained in this study had good development outlook.