In vitro toxicity of various classes of test agents using the neutral red assay on a human three-dimensional physiologic skin model |
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Authors: | Dennis Triglia Sonia Sherard Braa Christine Yonan Gail K Naughton |
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Institution: | (1) Marrow-Tech, Inc., 10933 North Torrey Pines Road, 92037 La Jolla, California |
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Abstract: | Summary A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human
neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro
toxicity of test agents from various classes. Utilizing a slight modification of the published neutral red viability assay
for endpoint determination, we have assayed and obtained dose-dependent toxicity curves for test agents from the following
classes: detergents (n=15), alcohols (n=5), metal chlorides (n=10), perfumes and colognes (n=5), shampoos (n=4), conditioners (n=3), moisturizers (n=3), pesticides (n=3), and antimicrobial preservatives (n=4). Limited comparisons to in vivo ocular irritancy data with alcohols and detergents are encouraging. We have demonstrated
the utility of this metabolically active dermal substrate containing naturally secreted collagen and other extracellular matrix
proteins along with the neutral red viability assay for assessing the toxicity of a number of test agents from a variety of
different classes with broad industrial applications. |
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Keywords: | in vitro toxicity neutral red assay 3-D skin model fibroblasts keratinocytes |
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