IL-7-mediated protection of pro and pre-B cells from the adverse effects of corticosterone |
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Authors: | Laakko Tonya Schwartz Richard C Fraker Pamela J |
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Institution: | Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA. laakko@med.unc.edu |
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Abstract: | The studies herein demonstrate that Interleukin-7 (IL-7) promotes survival of murine pro- and pre-B cells against stress levels of corticosterone (Cs). In short-term, 16-h, bone marrow cultures IL-7 abrogated Cs-induced apoptosis and cell cycle arrest in pro-B cells by decreasing apoptosis 60% and completely restoring the cell cycle. IL-7 also reduced Cs-induced apoptosis by 36% in pre-B cells and 24% in IgM(+) B cells, but did not restore deficits in the cell cycle. Among pro- and pre- B cells, substantial protection against high, pharmacological, levels of Cs was also provided by IL-7. Interestingly, stem cell factor, while reducing spontaneous apoptosis in pro-B cells, did not protect against Cs-induced death, either alone or with IL-7. In conclusion, IL-7 has potential immunotherapeutic value since it provides substantial protection to pro- and pre-B cells against the adverse effects of Cs. |
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Keywords: | Apoptosis Bone marrow Corticosterone Cell cycle Flow cytometry Glucocorticoid Interleukin-7 Lymphopoiesis Stem cell factor |
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