Plasminogen activator: Isolation and purification from Lymphosarcoma of ascites bearing mice |
| |
Authors: | M W Nulkar Rukmini Darad M Subramanian A R Pawse |
| |
Institution: | (1) Biochemistry Division, Bhabha Atomic Research Centre, Trombay, 400 085 Bombay, India |
| |
Abstract: | Plasminogen activator secreted by lymphosarcoma (ascites) of mice was purified up to 163-fold by ammonium sulphate fractionation
at 35% saturation and chromatography on p-aminobenzamidine-Sepharose 4B. The purified activator contained specific activity
of 9980 IU/mg. The plasminogen activator displayed homogeneity by polyacrylamide slab gel electrophoresis and high performance
liquid chromatography. The activator consisted of a single polypeptide chain with an apparent molecular weight of 66,000 daltons
as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis under reducing conditions as well as gel filtration
on Sephadex G-100. Distinct differences between this activator and urokinase were discernible in respect of specific activities,
fibrin affinity and immunochemical properties. The lymphosarcoma activator appears to be of tissue-type origin since it showed
gross similarity to standard tissue plasminogen activator in terms of modes of binding to fibrin and immunological attributes. |
| |
Keywords: | Plasminogen activator urokinase fibrinolysis Fibrin lymphosarcoma |
本文献已被 SpringerLink 等数据库收录! |