Low [3H]Cytochalasin B Binding in the Cerebral Cortex of Newborn Rat |
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Authors: | Anne M Morin Barney E Dwyer Denson G Fujikawa Claude G Wasterlain |
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Institution: | Veterans Administration Medical Center, Sepulveda, and Department of Neurology and Brain Research Institute, University of California School of Medicine, Los Angeles, California, U.S.A. |
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Abstract: | The concentrations of glucose transporter in the cerebral cortex and brainstem of neonatal (4–7 days old) and adult rats were measured using 3H]cytochalasin B binding. There was significantly lower binding in neonatal cortex (1.9 ± 0.7 pmol/mg protein) compared to adult (8.9 ± 2.5 pmol/mg protein). Scatchard analysis indicates this difference is due to a lower Bmax (neonate, 9.7 pmol/mg protein; adult, 18.6 ± 1.3 pmol/mg protein). Measurement of 3H]cytochalasin B binding in microvessels prepared from cortex of adult (28.1 ± 3.5 pmol/mg protein) and neonate (12.8 ± 1.9 pmol/mg protein) indicates a lower binding in the microvasculature of neonates, whereas no such difference was seen in the binding in microvessels prepared from adult and neonatal brainstem (adult, 11.8 ± 2.3 pmol/mg protein; neonate, 9.4 ± 2.7 pmol/mg protein). In both adult and neonate brain, there is an enrichment of glucose transporters in the microvasculature. |
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Keywords: | [3H]Cytochalasin B binding Glucose transporter Rat cerebral cortex |
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