Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer) |
| |
| Authors: | W F van Hooft O Hanotte P W Wenink A F Groen Y Sugimoto H H Prins A Teale |
| |
| Affiliation: | Wageningen Agricultural University, Department of Environmental Sciences, Wageningen, The Netherlands. |
| |
| Abstract: | The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5.0 (SE = 0.2) and the mean heterozygosity per marker was 0.61 (SE = 0.03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo. |
| |
| Keywords: | African buffalo bovine interspecific amplification microsatellites polymorphism Syncerus caffer |
|
