Culture of cells in beem capsules: A new technique for electron microscopic study of monolayer cultures

Summary A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules. BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell to substratum contacts with a minimum of stress and damage in the preparative steps for electron microscopy. This work was supported by grant AM 17631 from the National Institute of Arthritis, Metabolism, and Digestive Diseases, grant CA 11339 from the National Cancer Institute. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care.