A simple method for preparing thin (10 microM) histological sections of undecalcified plastic embedded bone with implants |
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Authors: | H B van der Lubbe C P Klein K de Groot |
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Affiliation: | Department of Biomaterials Sciences, ACTA, Free University, Amsterdam, The Netherlands. |
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Abstract: | A simple method for preparing undecalcified thin sections of bone with implants has been developed. After exposing a surface of bone and implant in a plastic block by sawing thick sections, the surface is stained prior to making a thin section. A glass coverslip is affixed with a thin layer of cement to the stained surface to stabilize the tissue and implant during sectioning. A mixture of glycerine and water is used as a coolant and lubricant. The orientation in situ is preserved allowing demonstration of bone architecture and cells, and the tissue-implant interface. |
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