药用植物川东獐牙菜的组织培养

针对川东獐牙菜野生资源受到严重破坏的情况 ,系统地探讨了通过组织培养为手段进行人工繁殖的方法 ,旨在为川东獐牙菜的保护提供坚实的理论依据。研究结果表明 :在所有的实验方案中 ,幼茎和老叶是理想的外植体材料。对叶片来说 ,较适宜的诱导愈伤组织的激素组合是 Zt1.0 m g/L +NAA 0 .0 5m g/L +IBA0 .0 5mg/L、BA 0 .5m g/L+2 ,4 - D0 .5mg/L或 BA 0 .2 m g/L+2 ,4 - D0 .2 mg/L+IBA0 .1m g/L ,对茎段来说 ,较适宜的诱导愈伤组织的激素组合是 BA0 .0 5m g/L+kt0 .0 5mg/L+IBA0 .0 5m g/L;诱导不定芽的适宜激素组合是 BA2 .0 mg/L+N AA0 .0 5mg/L或 BA 2 .0 mg/L+IBA 0 .1m g/L ;而根的诱导则是 MS+BA 0 .0 5mg/L+kt1.0 mg/L+N AA0 .1m g/L或 MS+BA1.0 m g/L+kt0 .3mg/L +IA A0 .5mg/L培养基上进行。

Tissue culture of medical plant Swertia davidii Franch

In order to protect the natural resources of Swertia davidii Franch. which has been destroyed seriously, the method of artificial propagation by way of tissue culture has been systematically researched. The results showed that the mature stems and the immature leaves are the best material explants in speeding propagation in all of the experiments. As for leaves, the suitable phytohormone compositions to induct callus is Zt1.0 mg/L+NAA0.05 mg/L?BA0.5 mg/L+2,4-D0.5 mg/L+IBA0.05 mg/L or BA0.2 mg/L+2,4-D0.2 mg/L+IBA0.1 mg/L. As for stems, the suitable phytohormone compositions to induct callus is BA0.05 mg/L+kt0.05 mg/L+IBA0.05 mg/L. The suitable phytohormone compositions to induct adventitious buds is BA2.0 mg/L+NAA0.05 mg/L or BA2.0 mg/L+IBA0.1 mg/L, and that of induct roots is MS+BA0.05 mg/L+kt1.0 mg/L+NAA0.1 mg/L or MS+BA1.0 mg/L+kt0.3 mg/L+IAA0.5 mg/L.