| RNA干扰抑制SiHaB2细胞中Bcl-2基因的表达 |
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| 引用本文： | 林学红 傅更锋 郭丹 刘新卷 王建军 徐根兴. RNA干扰抑制SiHaB2细胞中Bcl-2基因的表达[J]. 细胞生物学杂志, 2005, 27(5): 578-584 |
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| 作者姓名： | 林学红 傅更锋 郭丹 刘新卷 王建军 徐根兴 |
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| 作者单位： | [1]南京大学生命科学学院,南京210093 [2]江苏省基因药物工程技术研究中心,南京210049 |
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| 摘 要： | Bcl-2基因作为细胞凋亡的一个潜在抑制剂调节细胞的死亡，抑制恶性肿瘤细胞中Bcl-2基因的表达可促进肿瘤细胞的凋亡。采用RNA干扰技术，合成了含有21个核苷酸的小双链干扰RNA（siRNA．Bcl-2），并构建了含有19个核苷酸基因的质粒载体（pSilencer2．1-U6-Bcl-2），把合成的siRNA．Bcl-2和pSilencer2．1-U．Bcl-2分别转导入Bcl-2高表达的细胞株SiHaB2中，通过Western印迹检测，免疫荧光法检测及DNA梯（ladder）检测，可观察到在导入siRNA．Bcl-2和pSilencer2．1-U．Bcl-2的SiHaB2细胞被培养72h后，可以明显抑制Bcl-2蛋白的表达。
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| 关 键 词： | RNA干扰技术 SiHaB2细胞 Bcl-2基因 基因表达 细胞凋亡 |
| 收稿时间： | 2005-01-24 |
| 修稿时间： | 2005-04-25 |
Suppression of Bcl-2 Gene Expression with RNAi in SiHaB2 Cell |
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| Xue-Hong Lin, Geng-Feng Fu, Dan Guo, Xin-Juan Liu, Jian-Jun Wang, Gen-Xing Xu. Suppression of Bcl-2 Gene Expression with RNAi in SiHaB2 Cell[J]. Chinese Journal of Cell Biology, 2005, 27(5): 578-584 |
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| Authors: | Xue-Hong Lin Geng-Feng Fu Dan Guo Xin-Juan Liu Jian-Jun Wang Gen-Xing Xu |
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| Affiliation: | 1.School of Life Sciences, Nanjing University, Nanjing 210093, China; 2.Jiangsu Research Center for Gene Pharmaceutical Engineering and Technology, Nanjing 210049, China |
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| Abstract: | Bcl-2 gene, a latent inhibitor of the apoptosis, modulates the death of cells. To suppress the expression of Bcl-2 gene in malignant cells can promote the apoptosis of tumor cells. In this study, we have synthesized a small double-strand interference RNA (siRNA-Bcl-2) containing 21 nucleotides and constructed a plasmid (pSilencer2.1-U6-Bcl-2) containing 19 nucleotides with RNAi. Whereafter, we have transferred both of them separately into the SiHaB2 cells, which highly express Bcl-2 proteins. By the means of Western blot analysis, immunofluorescent analysis and DNA ladder analysis, we have observed that the expression of Bcl-2 proteins in the SiHaB2 cells transferred with siRNA-Bcl-2 or psilencer2.1-U6-Bcl-2 was notably inhibited. |
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| Keywords: | Bcl-2 gene apoptosis RNA interference gene silencing SiHaB2 cell |
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