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Polysulfide reductase and formate dehydrogenase from Wolinella succinogenes contain molybdopterin guanine dinucleotide |
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| Authors: | A. Jankielewicz R. A. Schmitz O. Klimmek A. Kröger |
| Affiliation: | (1) Institut für Mikrobiologie, J. W. Goethe-Universität, Marie-Curie-Strasse 9, D-60053 Frankfurt/Main, Germany;(2) Laboratorium für Mikrobiologie, Fachbereich Biologie der Philipps-Universität, Karl-von-Frisch-Strasse, D-35043 Marburg/Lahn, Germany;(3) Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043 Marburg/Lahn, Germany |
| Abstract: | Pterin derivatives were extracted from formate dehydrogenase and from polysulfide reductase of Wolinella succinogenes and converted to 6-carboxypterin. The amounts of 6-carboxypterin were consisted with the molybdenum content of the enzymes. The bis(carboxamidomethyl) derivatives of the cofactors showed absorption spectra that were identical with that of the corresponding molybdopterin guanine dinucleotide derivative (cam MGD). After hydrolysis of the derivatives with nucleotide pyrophosphatase in the presence of alkaline phosphatase, guanosine was formed together with a compound showing the properties of dephospho-bis(carboxamidomethyl)-molybdopterin. It is conluded that both formate dehydrogenase and polysulfide reductase of W. succinogenes contain molybdopterin guanine dinucleotide.Abbreviations MPT molybdopterin - MGD molybdopterin guanine dinucleotide - cam MPT bis(carboxyamidomethyl)-molybdopterin - cam MGD bis(carboxyamidomethyl)-molybdopterin guanine dinucleotide |
| Keywords: | Pterin derivatives Wolinella succinogenes Polysulfide reductase Formate dehydrogenase Molybdopterin guanine dinucleotide |
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