New development in the tritium labelling of peptides and proteins using solid catalytic isotopic exchange with spillover-tritium |
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Authors: | Yu. A. Zolotarev A. K. Dadayan E. V. Bocharov Yu. A. Borisov B. V. Vaskovsky E. M Dorokhova N. F. Myasoedov |
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Affiliation: | (1) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia, RU;(2) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia, RU;(3) A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia, RU |
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Abstract: | Summary. The mechanism of the reaction of high temperature solid state catalytic isotope exchange (HSCIE) of hydrogen in peptides with spillover-tritium at 140–180°C was analyzed. This reaction was used for preparing [3H]enkephalins such as [3H]DALG with specific activity of 138 Ci/mmol and [3H]LENK with specific activity of 120 Ci/mmol at 180°C. The analogues of [3H]ACTG4–10 with specific activity of 80 Ci/mmol, [3H]zervamicin IIB with specific activity of 70 Ci/mmol and [3H]conotoxin G1 with specific activity 35 Ci/mmol were produced. The obtained preparations completely retained their biological activity. [3H]Peptide analysis using 3H NMR spectroscopy on a Varian UNITY-600 spectrometer at 640 MHz was carried out. The reaction ability of amino fragments in HSCIE was shown to depend both of their structures and on the availability and the mobility of the peptide chain. The reaction of HSCIE with the β-galactosidase from Termoanaerobacter ethanolicus was studied. The selected HSCIE conditions allow to prepare [3H] β-galactosidase with specific activity of 1440 Ci/mmol and completely retained its the enzymatic activity. Received November 30, 2001 Accepted January 31, 2002 Published online December 18, 2002 Acknowledgments The work was supported by the Russian Foundation for Basic Research, grant 01-04-48519a. Authors' address: Dr. Yurii A. Zolotarev, Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, 123182, Moscow, Russia, Fax: +7 (095) 196-0221, E-mail: zolya@img.ras.ru Abbreviations: HSCIE, the reaction of high temperature solid state catalytic isotope exchange; HS, hydrogen spillover; 3H NMR, tritium nuclear magnetic spectroscopy; CtxG1, conotoxin G1; AchR, acetylcholine receptor; HF, Hartree-Fock ab initio quantum-chemical calculation method |
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Keywords: | : Tritium labelled Amino acids Peptides Enkephalins Spillover-hydrogen Isotope exchange Tritium NMR |
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