Growth characteristics of a continuous cell line from the velvetbean caterpillar,Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) |
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| Authors: | Peggy J Sieburth James E Maruniak |
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| Institution: | (1) Department of Entomology and Nematology, University of Florida, Bldg. 345, Archer Road Lab, 32611 Gainesville, Florida;(2) Present address: ARS, USDA, ABADRL, University Station, P.O. Box 3965, 82071 Laramie, WY |
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| Abstract: | Summary The cell line UFL-AG-286 was established from the embryos ofAnticarsia gemmatalis (Lepidoptera: Noctuidae). The cell line was characterized by isozyme analysis and from molecular weight determination of
the restriction endonuclease bands of the mitochondrial DNA. Cells seeded in 24-cluster wells had a doubling time of 5.9 d
when seeded at 2×105 cells ml and 6.7 d when seeded at 3×105 cells/ml.
This work was funded by U.S. Department of Agriculture grant 84-CRCR-1-1431. Florida Agricultural Experiment Station Journal
Series, no. 8181. |
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| Keywords: | insect cell line Lepidoptera Anticarsia gemmatalis embryo mitochondrial DNA isozymes |
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