The disease resistance response in pea is associated with increased levels of specific mRNAs |
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Authors: | Robert C. Riggleman Brian Fristensky Lee A. Hadwiger |
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Affiliation: | (1) Program in Molecular Biology, Princeton University, 99164-6430 Pullman, WA, U.S.A.;(2) Program in Genetics and Cell Biology, Washington State University, 99164-6430 Pullman, WA, U.S.A.;(3) Department of Plant Pathology, Washington State University, 99164-6430 Pullman, WA, U.S.A. |
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Abstract: | A cDNA library was constructed using poly(A)+RNA fromPisum sativum which had been treated for 8 h with the fungusFusarium solani f. sp.phaseoli. Two thousand four hundred recombinant colonies were screened by differential colony hybridization using32P-labelled cDNAs prepared from RNA extracted from either noninoculated or inoculated pea tissue. cDNA clones were then selected, which showed greater hybridization with cDNA prepared from pea RNA 8 h post-inoculation than with a cDNA probe from 0 h. Seven distinct hybridization classes were chosen for further study. Northern blot analyses of total cellular RNAs inoculated for 16 h with eitherF. solani phaseoli or water demonstrated that each cDNA clone selected represents an mRNA species which increases substantially in abundance during infection. Results of3H-uridine pulse-labelling experiments suggested that enhanced synthesis is at least partially responsible for the accumulation of the fungus-inducible mRNAs which hybridized with the clones. |
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