Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome |
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| Institution: | 1. Cambridge Center for Proteomics, Milner Therapeutics Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Puddicombe Way, Cambridge CB2 0AW, UK;2. Humboldt University Berlin, IRI Life Sciences, Philippstr. 13 10115 Berlin, Germany;3. MRC Toxicology Unit, University of Cambridge, Leicester, LE1 7BH, UK;4. German Cancer Research Center, Im Neuenheimer Feld 581, Heidelberg, Germany;5. Heidelberg University, Medical Faculty, Im Neuenheimer Feld 672, Heidelberg, Germany |
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| Abstract: | Protein–RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limiting their application. With the advent of organic–aqueous phase separation–based methods, we now have technologies that efficiently enrich the complete suite of RBPs and enable quantification of RBP dynamics. These flexible approaches to study RBPs and their bound RNA open up new research avenues for systems-level interrogation of protein–RNA interactions. |
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