Anticancer efficacies of persicogenin and homoeriodictyol isolated from Rhus retinorrhoea |
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| Affiliation: | 1. Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, 605 008, India;2. Department of Botany, Siddha Clinical Research Unit, Central Council for Research in Siddha, Palayamkottai, Tamil Nadu, 627 002, India;3. Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia;4. Department of Plant Pathology, University of Minnesota, Twin cities, Saint Paul, 55108, United States;5. Department of Life Sciences, Presidency University, College Street, Kolkata, 700 073, India;1. Chair for DNA Research, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;2. Chemistry Department, Faculty of Science, Taibah University, Medina (Yanbu), Saudi Arabia;3. Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, Republic of Korea;4. Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Republic of Korea;5. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia;6. Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia |
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| Abstract: | Herein we examined two flavanones (persicogenin and homoeriodictyol) isolated from Rhus retinorrhoea to elucidate the mechanism of their anticancer effects in MCF-7, HeLa, and HT-29 cells. Based on the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] (MTT) cytotoxicity data of persicogenin (500 μg/ml) caused a 58.1 % reduction in HT-29 survival while homoeriodictyol (500 μg/ml) caused a 51.9 %, 66.7 % and 76.2 % reductions in MCF-7, HeLa and HT-29 cell survival, respectively. The neutral red uptake (NRU) assay revealed 53.6 %, 53.9 %, 58.8 % and 83.0 %, 87.7 %, 66.7 % reductions in MCF-7, HeLa, and HT-29 cell survival following persicogenin and homoeriodictyol (500 μg/ml) treatment, respectively. Moreover, the intracellular reactive oxygen species (ROS) was significantly enhanced and dysfunction of mitochondrial membrane potential (ΔΨm) confirmed the mitochondrial injury in all cell types by the flavanones. MCF-7, HeLa, and HT-29 cells exposed to persicogenin and homoeriodictyol (500 μg/ml) had showed 42.5 %, 63.1 %, 62.3 % and 30.7 %, 30.2 %, 23.8 % cells in the sub G1 apoptotic phase. The persicogenin- and homoeriodictyol-treated cell lines had upregulated expressions of p53, caspase-3, caspase-9, bax, and superoxide dismutase 1 (SOD1) genes. Such findings provide novel insight into the comparative anti-cancer efficacy of persicogenin and homoeriodictyol, signifying their promising clinical applications as cancer treatments and their application as bioactive therapeutic agents. |
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| Keywords: | Homoeriodictyol Persicogenin Flavanone Anti-cancer Apoptosis |
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