Bioactivity-guided isolation of antimicrobial metabolite from Xylaria sp. |
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| Institution: | 1. Microbial Drugs Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysuru, 570 006, Karnataka, India;2. Department of Microbiology, Yuvaraja’s College (Autonomous), University of Mysore, Mysuru, 570 005, Karnataka, India;3. Department of Studies in Organic Chemistry, Manasagangotri, University of Mysore, Mysuru, 570 006, Karnataka, India;4. Department of Biochemistry, Indian Institute of Science, Bengaluru, 560012, Karnataka, India;1. Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, PR China;2. Hefei Preschool Education College, Hefei, 230013, PR China;1. Institute of Infection, Immunity and Inflammation, University of Glasgow, United Kingdom;2. Department of Biochemistry, Faculty of Natural Sciences, Kogi State University, Nigeria;3. Department of Chemistry, College of Science, University of Agriculture, Makurdi, Nigeria;4. Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom;5. Department of Biochemistry, College of Health sciences, University of Nigeria, Nigeria;1. Centre of Research for Development and Department of Environmental Sciences, University of Kashmir, Srinagar 190006, India;2. Department of Botany, University of Kashmir, Srinagar 190006, India;1. CNRS, Université Paris-Sud, Institut de Chimie des Substances Naturelles, 1 avenue de la Terrasse, 91198 Gif-sur-Yvette cedex, France;2. CNRS/Sorbonne Universités, LBBM, Observatoire Océanologique, 66650 Banyuls-sur-mer, France;1. Microbial Drugs Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysore, 570006, Karnataka, India;2. Department of Microbiology, Yuvaraja’s College (Autonomous), University of Mysore, Mysuru, 570 005, Karnataka, India;3. Department of Biochemistry, Indian Institute of Science, Bengaluru, 560 012, Karnataka, India |
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| Abstract: | Symbiotic plant-microbe metabolic interactions not only have beneficial effects on plants but also contribute to rich, unmatched and complex chemical biodiversity with biological potential. Systematic delineated bioprospecting of fungal diversity associated with Ficus pumila Linn (Moraceae) for antimicrobial metabolite revealed Xylaria sp. FPL-25(M). The present study describes bioactivity guided fractionation prioritized for antimicrobial potential. Thus, chemical investigation of culture broth of Xylaria sp. FPL-25(M) by bioactivity guided fractionation with spectroscopic techniques revealed bioactive metabolite xylobovide-9-methyl ester. The xylobovide-9-methyl ester exhibited broad-spectrum antimicrobial activity. However, Gram-positive bacteria and fungi were more susceptible than Gram-negative bacteria. The present study results represent bioassay-based screening strategy which facilitates rapid, efficient and reliable approach for endophytic strain prioritization for novel bioactive molecules. |
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| Keywords: | Mycoendophyte Bioautography Hyphenated spectroscopy Antimicrobial |
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