Poly(ethylene terephthalate) (PET) degradation by Yarrowia lipolytica: Investigations on cell growth,enzyme production and monomers consumption |
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| Affiliation: | 1. Biochemical Engineering Department, School of Chemistry, Federal University of Rio de Janeiro. Av. Athos da Silveira Ramos, 149. Ilha do Fundão., Rio de Janeiro, CEP: 21941-909, Brazil;2. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France;3. Biotechnology Division, R&D Center (Cenpes), PETROBRAS, Av. Horácio Macedo, 950. Ilha do Fundão, Rio de Janeiro, CEP: 21941-915, Brazil;1. Biotechnology Division, Research and Development Center (Cenpes), PETROBRAS, Av. Horácio Macedo, 950. Ilha do Fundão, Rio de Janeiro, 21941-915, Brazil;2. Falcão Bauer. R. Aquinos, 111. Água Branca, São Paulo, 05036-070, Brazil;1. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China;1. Biotechnology Division, Research and Development Center (Cenpes), PETROBRAS, Av. Horácio Macedo, 950, 21941-915, Rio de Janeiro, Brazil;2. Falcão Bauer, R. Aquinos, 111, Água Branca, São Paulo, 05036-070, Brazil;3. Institute of Macromolecules Professor Eloisa Mano (IMA), Federal University of Rio de Janeiro (UFRJ), Av. Horácio Macedo, 2030, 21941-598, Rio de Janeiro, Brazil;4. Chemistry Division, Research and Development Center (Cenpes), PETROBRAS, Av. Horácio Macedo, 950, 21941-915, Rio de Janeiro, Brazil;1. Biotechnology Division, Research and Development Center, PETROBRAS, Av. Horácio Macedo, 950, Ilha do Fundão, Rio de Janeiro, 21941-915, Brazil;2. Falcão Bauer. R. Aquinos, 111. Água Branca, São Paulo, 05036-070, Brazil;3. Chemistry Division, Research and Development Center (Cenpes), PETROBRAS, Av. Horácio Macedo, 950, 21941-915, Rio de Janeiro, Brazil |
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| Abstract: | The development of technologies for poly(ethylene terephthalate) (PET) depolymerization, such as biocatalysis, has been pointed as a very promising alternative to chemical hydrolysis processes. This work aims to understand the behavior of Yarrowia lipolytica, a robust yeast for diverse applications, in the presence of molecules from the PET production chain such as monoethylene glycol (MEG), terephthalic acid (TPA), bis (2-hydroxyethyl) terephthalate (BHET), PET oligomers, amorphous PET and post-consumer PET. The yeast was cultivated in rich media with and without glucose addition, in order to compare monomers release or consumption. TPA and MEG were consumed more intensely in the absence of glucose. The addition of the diester BHET yielded a 3-fold increased lipase production both at 160 rpm (118 U/L) and 250 rpm (385 U/L). In addition, 250 rpm agitation also provided a higher consumption of TPA (26% increase). The mono(2-hydroxyethyl) terephthalate (MHET) was the main intermediate released during polymer hydrolysis, followed by TPA and BHET. Thus, the use of Yarrowia lipolytica, which is capable of catalyzing the PET hydrolysis is of great potential to reduce the environmental impacts caused by unappropriated disposal of packages. |
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| Keywords: | Biocatalysis Depolymerization Lipase PET recycling Poly(ethylene terephthalate) |
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