Enhanced production of cellulase from a novel strain Trichoderma gamsii M501 through response surface methodology and its application in biomass saccharification |
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| Affiliation: | 1. Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119 Tamil Nadu India;2. Department of Biotechnology, Alagappa University, Science Campus, Karaikudi 630003, Tamil Nadu India;1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, China;2. The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, Heilongjiang, China;1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China;1. Bioengineering Department, Manisa Celal Bayar University, Yunusemre, Manisa, Turkey;2. Department of Mechanical Engineering, Izmir Katip Çelebi University, Cigli, Izmir, Turkey;1. National Centre for Catalysis Research and Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India;2. National Centre for Catalysis Research and Department of Chemistry, Indian Institute of Technology-Madras, Chennai, 600 036, India;3. School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M13 9PL, United Kingdom;4. Department of Chemical and Process Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom;1. Department of Biochemistry and Chemical Technology, IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil;2. Department of Biology, IBILCE/UNESP–Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil;3. Department of Chemistry and Environmental Sciences, IBILCE/UNESP – Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil;4. Department of Organic Chemistry, IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil;5. Department of Biochemistry and Microbiology, IB/UNESP–Univ Estadual Paulista, Avenida 24A, 1515, 13506-900, Rio Claro, São Paulo State, Brazil |
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| Abstract: | Cellulolytic enzymes produced by Trichoderma sp. have attracted interest in converting the biomass to simple sugars in the production of cellulosic ethanol. In this work, a novel cellulolytic strain M501 was isolated and identified as T. gamsii by sequencing the ITS rDNA region. The production of cellulase (CMCase) by T. gamsii M501 was enhanced by employing statistical methods. The strain grown in the optimized production medium composed of mineral salts, microcrystalline cellulose (13.7 g/l), tryptone (4.8 g/l) and trace elements (2 mL/l) at pH 5.5 and 28 °C for 72 h produced a maximum CMCase of 61.3 U/mL. The optimized production medium also showed the other enzyme activity of FPU (2.6 U/mL), β-glucosidase (2.1 U/mL), xylanase (681 U/mL) and β- xylosidase (0.6 U/mL). The crude cellulase cocktail produced by T. gamsii M501 efficiently hydrolyzed alkali pretreated sugarcane bagasse with glucose and xylose yield of 78 % and 74 % respectively at 10 % solid loading. This study is the first of its kind research on biomass saccharification using T. gamsii cellulase cocktail. Therefore, the novel strain T. gamsii M501 would be useful for further development of an enzyme cocktail for cellulosic ethanol production. |
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| Keywords: | Biomass saccharification Cellulase Endoglucanase Plackett-Burman Central composite design |
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