The effect of ultrasonication on enzymatic hydrolysis of chitin to N-acetyl glucosamine via sequential and simultaneous strategies |
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| Institution: | 1. Department of Radiology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China;2. Department of Stomatology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China;1. Department of Mechanical and Electro-Mechanical Engineering, National I-Lan University, Yilan, Taiwan;2. Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia;3. Centre For Automotive Research and Tribology, Indian Institute of Technology, Delhi, India;4. Department of Mechanical Engineering, and Center for Engineering and Sustainable Development Research, De La Salle University, Philippines;5. School of Information, Systems and Modelling, Faculty of Engineering and Information Technology, University of Technology Sydney, NSW 2007, Australia;1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China;1. Department of Emergency, China-Japan Union Hospital of Jilin University, Changchun 130033, China;2. Department of Respiratory Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China;1. INRS-Institut Armand-Frappier, Université du Québec, 531 Boul. des Prairies, Laval, Québec H7V 1B7, Canada;2. PROTEO, The Québec Network for Research on Protein Function, Engineering, and Applications, 1045 Avenue de la Médecine, Université Laval, Québec, Québec, G1V 0A6, Canada;3. GRASP, The Groupe de Recherche Axé sur la Structure des Protéines, 3649 Promenade Sir William Osler, McGill University, Montréal, Québec, H3G 0B1, Canada |
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| Abstract: | In this study, sequential and simultaneous strategies of ultrasonication (ultrasonic bath) were investigated to enhance enzymatic production of N-acetyl glucosamine (GlcNAc) from chitin powder. For the sequential strategy, the ultrasonic caused chitin powder to a visible fleecy structure, and Scanning electron microscopy (SEM) showed the surface of the treated chitin had a fiber-like structure with a diameter of 50 ? 200 nm. Moreover, Fourier transform infrared spectroscopy (FTIR), Element analysis (EA), and X-ray diffraction (XRD) revealed that the crystallinity of the chitin decreased with little deacetylation. The simultaneous strategy is a one-pot treatment and enzymatic hydrolysis of chitin. The concentration of GlcNAc was 2.65 g/L for the strategy, which was 1.18- and 5.0-fold higher than the sequential strategy (2.25 g/L) and untreated chitin (0.53 g/L), respectively. In conclusion, this approach provided an efficient and environmentally friendly method for reducing the crystallinity of chitin and enhancing its enzymatic hydrolysis. |
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| Keywords: | Ultrasonication Chitin GlcNAc Chitinase Pretreatment |
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