Optimization of cultivation conditions for efficient production of carotenoid-rich DHA oil by Schizochytrium sp. S31 |
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| Affiliation: | 1. Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China;2. Qianxi County Bureau of Agriculture and Animal Husbandry, 40 Lixini Avenue, Tangshan 064300, China;1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People’s Republic of China;2. School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People’s Republic of China;3. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, No. 5 Xinmofan Road, Nanjing 210009, People’s Republic of China;4. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), No. 5 Xinmofan Road, Nanjing 210009, People’s Republic of China;1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Wilmar Biotechnology Research Development Center Ltd, Shanghai, China;1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China;2. Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan |
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| Abstract: | The high carotenoid content in docosahexaenoic acid (DHA) oil may serve as an avenue to avoid DHA oxidation during storage. To obtain carotenoid-rich DHA oil from Schizochytrium sp. S31, the effects of several key medium components were investigated. Shake-flask experiment revealed that in optimal conditions, glycerol was used as the carbon source with 0.25 g/L of KH2PO4 and a C/N ratio of 75:20. Fed-batch cultivation further showed that a high lipid accumulation (30.81 g/L) with 497.56 μg/g carotenoid and 12.24 g/L DHA was obtained under the optimized conditions. The increased carotenoid content resulted in improved defense against environmental stress, as indicated by the catalase (CAT) and superoxide dismutase (SOD) activities. Further transcriptome analysis demonstrated that the synthesis pathway of β-carotene was enhanced during the lipid turnover stage, which suggested that more carotenoids were produced to reduce oxidative stress. This study presented an efficient strategy for DHA oil production with high carotenoid content. |
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| Keywords: | Carotenoid Docosahexaenoic acid AStaxanthin Oxidative stress |
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