Performance evaluation and microbial community analysis of the composite filler micro-embedded with Pseudomonas putida for the biodegradation of toluene |
| |
| Institution: | 1. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China;2. School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China;3. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;1. School of Life Sciences, Ludong University, Yantai 264025, China;2. School of Agriculture, Ludong University, Yantai 264025, China;1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China;2. Department of Biosystems Engineering and Soil Science, The University of Tennessee, 2506 E.J. Chapman Drive, Knoxville, TN, 37996, USA;1. Research Group EnVOC, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium;2. X-ray Microspectroscopy & Imaging Group XMI, Faculty of Sciences, Ghent University, Krijgslaan 281 S12, 9000, Ghent, Belgium;1. School of Environment, Harbin Institute of Technology, Harbin 150001, PR China;2. School of Environmental Science and Engineering, College of Engineering, Southern University of Science and Technology, Shenzhen 518055, PR China;3. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China;4. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, PR China;5. State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, PR China;6. Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, PR China;1. Department of Environmental Health Engineering, Environmental Science, and Technology Research Center, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;2. Department of Environmental Health Engineering, Faculty of Health, Environmental Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran;3. Department of Biostatistics, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran |
| |
| Abstract: | The composite filler micro-embedded with Pseudomonas putida (P. putida) was prepared and the biodegradation performance of the filler was evaluated in a biofilter. Five phases were set up to evaluate the performance of the biofilter under different toluene inlet loadings and transient shock loadings. In particular, the microbial community structure in the biofilms and fillers was measured by sequence analysis of the 16S rRNA gene. The results show that the biofilter packed with the composite fillers was suitable for the biodegradation of toluene. The biofilter could start up quickly with high removal efficiency (RE), and remain above 90 % RE when the empty bed residence time (EBRT) was 18 s and the inlet loading rates were not higher than 41.4 g/(m3·h). Moreover, the biofilter could tolerate substantial transient shock loadings. The high removal efficiency and elimination capacity contributed to rich bacterial communities for the efficient degradation of toluene. The dominant microbial communities at the phylum level were mainly Firmicutes, Actinobacteria and Proteobacteria. It is noteworthy that the abundance of Bacteroidetes at phylum level and Chungangia and Stenotrophomonas at genus level increased significantly during the re-start period. |
| |
| Keywords: | Microbial community Biofilter Toluene Composite filler |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
