Genetic diversity of groEL and msp4 sequences of Anaplasma ovis infecting camels from Tunisia |
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| Institution: | 1. Service de Microbiologie et Immunologie, Ecole Nationale de Médecine Vétérinaire, Université de Manouba, 2020 Sidi Thabet, Tunisie;2. Service de Parasitologie, Ecole Nationale de Médecine Vétérinaire, Université de Manouba, 2020 Sidi Thabet, Tunisie;3. Ministère de la Défense Nationale, Direction Générale de la Santé Militaire, Service Vétérinaire, Tunis, Tunisie;4. Institut National Agronomique de Tunis, Université de Carthage, Tunisie;1. Division of Parasitology, ICAR- Indian Veterinary Research Institute, Izatnagar, U.P., India;2. Department of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;3. Department of Veterinary Medicine, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;4. ICAR- IVRI, Eastern Regional Station, Kolkata W.B., India;5. Division of Physiology and Climatology, ICAR-IVRI, Izatnagar, U.P., India;6. Division of Biological Standardization, ICAR-IVRI, Izatnagar, U.P., India |
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| Abstract: | To date, no information is available regarding the infection of camels (Camelus dromedarius) by Anaplasma ovis in North African region. Several animal species can be infected by A. ovis which further complicates its natural infection cycle. In this paper, we investigated the occurrence and the genetic diversity of A. ovis in camels and ticks collected from them in Tunisia and the risk factor analysis. Camel blood samples (n = 412) and tick (n = 300) samples, identified as Hyalomma dromedarii (n = 149, 49.6%), H. impeltatum (n = 142, 47.3%) and H. excavatum (n = 9, 3%), were analyzed by conventional PCR followed by the sequencing of msp4 and groEL genes. A. ovis DNA was identified in five camels (1.2%), but not in infesting ticks (0%). The microscopic examination revealed the specific infection of camel erythrocytes by Anaplasma inclusions. The msp4 and groEL typing confirmed the natural infection of camels by A. ovis and revealed two different msp4 genotypes earlier detected in Tunisian small ruminants and their infested ticks, and five different and novel groEL genetic variants forming a separately sub-cluster within A. ovis cluster. The occurrence of different A. ovis strains specific to camels associated with a low prevalence of this Anaplasma species in camels may enrich knowledge regarding the distribution and the transmission cycle of this bacterium in arid and Saharan areas of Tunisia. |
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