Novel Single-step Pretreatment of Steam Explosion and Choline Chloride to De-lignify Corn Stover for Enhancing Enzymatic Edibility |
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| Affiliation: | 1. State Key Laboratory of Biochemical Engineering, Beijing Key Laboratory of Biomass Refining Engineering, Institute of Process and Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China;2. University of Chinese Academy of Sciences, Beijing, 100049, PR China;1. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan, 320, Taiwan;2. Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia;1. Industrial Bio-materials Research Center, KRIBB, Daejeon 34141, Republic of Korea;2. Department of Biological Sciences, Andong National University, Andong 36729, Republic of Korea;3. Insect Biotech Co. Ltd., Daejeon 34054, Republic of Korea;1. School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso, Chile;2. Department of Biology, Faculty of Chemistry and Biology, Universidad de Santiago de Chile (USACH), Santiago, Chile;1. Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India;2. School of Biotechnology and Bioinformatics, D.Y. Patil University, Navi Mumbai, India;1. College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China;1. College of Forestry and Landscape Architecture, Guangdong Province Research Center of Woody Forage Engineering Technology, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China;2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | It is important to develop efficient and economically feasible pretreatment methods for lignocellulosic biomass, to increase annual biomass production. A number of pretreatment methods were introduced to promote subsequent enzymatic hydrolysis of biomass for green energy processes. Pretreatment with steam explosion removes the only xylan at high severity but increases lignin content. In this study, corn stover soaked in choline chloride solution before the steam explosion is economically feasible as it reduced cost. Enzymatic hydrolysis of de-lignified corn stover is enhanced by combinatorial pretreatments of steam explosion and choline chloride. Corn stover pretreated with choline chloride at the ratio of 1:2.2 (w/w), 1.0 MPa, 184 °C, for 15 min efficiently expelled 84.7% lignin and 78.9% xylan. The residual solid comprised of 74.59% glucan and 7.51% xylan was changed to 84.2% glucose and 78.3% xylose with enzyme stacking of 10FPU/g. This single-step pretreatment had ∼ 4.5 and 6.4 times higher glucose yield than SE-pretreated and untreated corn stover, respectively. Furthermore, SEM, XRD and FTIR indicated the porosity, crystalline changes, methoxy bond-cleavage respectively due to the lignin and hemicellulose expulsion. Thus, the released acetic acid during this process introduced this novel strategy, which significantly builds the viability of biomass in short pretreatment time. |
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| Keywords: | Choline chloride Enzymatic Edibility Steam explosion Untreated Corn Stover |
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