Surfactant induces ROS-mediated cell membrane permeabilization for the enhancement of mannatide production |
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| Institution: | 1. College of Biological Engineering, Henan University of Technology, 450001, Zhengzhou, China;2. National Engineering Laboratory for Wheat & Corn Further Processing, Henan University of Technology, 450001, Zhengzhou, China;3. School of Chemistry and Chemical Engineering, Henan Normal University, 453001, Xinxiang, China;4. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China;1. Key Laboratory of Textile Fibers and Products, Ministry of Education, P. R. C, College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, China;2. Third Institute of Oceanography, Ministry of Natural Resources, P. R. C, Xiamen 361005, China;3. High-Tech Organic Fibers Key Laboratory of Sichuan Province, Sichuan Textile Research Institute, Chengdu 610072, China;1. School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China;2. School of Energy and Environment (SEE), City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China;3. Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen, 518055, China;4. School of Civil and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China;1. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, No. 68, Xuefu South Road, Changqing Garden, Wuhan, 430023, P.R. China;2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No. 44, Xiaohongshan, Wuhan 430071, P.R. China;3. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190 P.R. China;1. School of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China;2. Roy J. Carver Dept. of Biochemistry, Biophysics & Molecular Biology, Iowa State University, 3192 Molecular Biology Building, Ames, IA, 50011, United States;3. College of Engineering, Department of Mechanical Engineering, Iowa State University, Ames, IA, 50011, United States;1. Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, ChangChun, Jilin, 130031, China;2. School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Malaysia;3. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia;4. Department of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia;5. Department of Oral & Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia;1. State Key Laboratory of Food Nutrition and Safety, Institute of Health Biotechnology, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China;2. College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China;3. College of Biological and Environmental Engineering, Binzhou University, Binzhou, Shandong, 256603, People’s Republic of China |
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| Abstract: | A surfactant was a substance that had an important influence on the excretion of intracellular substances. In this work, it was found that cetyltrimethylammonium bromide (CTAB) inhibited cell viability but increased the mannatide production by optimizing the addition time. Results revealed that CTAB changed cell surface properties (cell surface hydrophobicity and Zeta potential was increased from 3% to 14% and ?14.5 mV to ?10.2 mV, respectively) and permeabilized cell membrane (intercellular ATP content was decreased from 28.599 μg/g to 9.737 μg/g while extracellular ATP content was increased from 33.051 μg/g to 82.809 μg/g; the concentrations of K+ and Ca2+ were increased to 3.9 mg/L and 2.1 mg/L, respectively; membrane potential was formed). Moreover, the images of scanning electron micrographs indicated distinct morphological changes and disruption on the surface of the cells. Further pyridinium iodides staining showed CTAB could induce cell apoptosis from 4.24% to 31% with increasing the relative intracellular reactive oxygen species (ROS) from 0.11% to 7.31%. It is the most noteworthy that the addition of CTAB increased the mannatide production to 1.46 g/L, 98.6% higher than that of untreated cells. Consequently, the utilization of CTAB for the preparation of mannatide provide theoretical foundation for the further large-scale production. |
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| Keywords: | CTAB Mannatide Permeability Membranes ROS |
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