Development and evaluation of different strategies for the clean synthesis of silver nanoparticles using Yarrowia lipolytica and their antibacterial activity |
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| Affiliation: | 1. Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran;2. Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran;3. Department of Microbiology, Faculty of Biological Science, Alzahra University, Tehran, Iran;1. Fraunhofer Institute of Interfacial and Bioprocess Engineering, Stuttgart, Germany;2. Fraunhofer Center for Chemical-Biotechnological Processes, Leuna, Germany;1. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan, 320, Taiwan;2. Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia;1. Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Engineering, China Agricultural University, Beijing 100083, China;1. School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso, Chile;2. Department of Biology, Faculty of Chemistry and Biology, Universidad de Santiago de Chile (USACH), Santiago, Chile;1. School of Environmental Studies, Jadavpur University, Kolkata 700 032, India;2. Department of Chemistry, Jadavpur University, Kolkata 700 032, India;3. Department of Chemical Engineering, National Institute of Technology, Mahatma Gandhi Road, Durgapur 713 209, India;4. Department of Chemical Engineering, McMaster University, 1280 Main St West, Hamilton, Ontario, L8S 4L8, Canada;1. Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India;2. Department of Botany, Pt. N.R.S. Govt College, Rohtak, 124001, Haryana, India;3. Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh, 123031, Haryana, India |
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| Abstract: | An environment-friendly, cheap method, biogenic synthesis of silver nanoparticles (AgNPs) is interesting as compared to physical and chemical synthesis methods. The aim of the present study was to utilize the inherent capability of Yarrowia lipolytica as a novel biocatalyst for green production of AgNPs using different strategies, including growing cells, resting cells, and cell-free extracts (CFE) under optimized reaction conditions. The produced AgNPs were evaluated with UV–vis spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared spectrometry. In the growing cells strategy, Y. lipolytica produced spherical AgNPs under the optimized conditions, 2.5 mM of silver ions, 7.5 g/l of yeast biomass, a temperature of 30 °C, a pH of 6, and a shaking rate of 50 rpm after 48 h. The sizes and monodispersity of the AgNPs in the resting cells strategy were better than those in the other two. However, the AgNPs were produced faster in the CFE strategy. The antibacterial activity and minimal inhibitory concentration of the AgNPs against certain Gram-positive and Gram-negative bacteria were determined by the agar well diffusion and broth microdilution methods. The AgNPs had a considerable antibacterial effect compared to chloramphenicol as a broad-spectrum antibiotic. |
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| Keywords: | Silver nanoparticle Optimization Antibacterial activity |
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