Enzyme-assisted extraction of a cup plant (Silphium perfoliatum L.) Polysaccharide and its antioxidant and hypoglycemic activities |
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| Institution: | 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China;2. Jilin Provincial Key Lab of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, China;3. Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China;4. Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China;5. Department of Animal Science, University of Arkansas, Fayetteville, 72701, USA;1. Department of Nursing Care, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China;2. Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China;3. Department of Orthopeadic Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China;1. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China;2. School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China;3. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;1. School of Life Sciences, Jilin University, Changchun 130021, PR China;2. School of Stomatology, Jilin University, Changchun 130000, PR China;1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;2. Jilin Provincial Key Lab of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China;3. Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;4. Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;5. Department of Animal Science, University of Arkansas, Fayetteville 72701, Arkansas, USA;6. Institute of Grassland Research, CAAS, Hohhot 010010, China |
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| Abstract: | The optimum enzyme-assisted extraction conditions of cup plant (Silphium perfoliatum L.) polysaccharides (CPP) were obtained by response surface methodology (RSM), and were found to be an enzymatic hydrolysis time of 66 min, a liquid-solid ratio of 22 mL/g, pH 5.1, and an enzyme-complex dosage of 1.59 %, allowing a CPP yield of 13.69 %. DEAE-52 cellulose and Sepharose CL-6B columns were used to purify the CPP. A purified polysaccharide component, CPP-1.2, was obtained and further analyzed for its characteristics, antioxidant properties, and hypoglycemic activity. The monosaccharide composition of CPP-1.2 was mannose, galacturonic acid, glucose, galactose, and arabinose with a molar percentage ratio of 2.24:16.34:44.17:24.28:12.97. The 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging abilities of CPP-1.2 (1.0 mg/mL) reached 95.20 % and 93.69 % that of ascorbic acid (1.0 mg/mL), respectively. Moreover, CPP-1.2 presented significantly higher inhibitory properties on the activities of α-amylase and α-glucosidase than acarbose at each concentration tested. These results suggest that CPP-1.2 possesses the potential to be used as an antioxidant and hypoglycemic agent. |
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| Keywords: | Polysaccharides Optimization Activity |
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