Directing cell catalysis of glucose to 2-keto-d-gluconic acid using Gluconobacter oxydans NL71 |
| |
| Affiliation: | 1. Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People’s Republic of China;2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People’s Republic of China;3. Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People’s Republic of China;4. Department of Engineering Technology, Biotechnology Program, College of Technology, University of Houston, Houston, TX 77004, USA;1. Industrial Bio-materials Research Center, KRIBB, Daejeon 34141, Republic of Korea;2. Department of Biological Sciences, Andong National University, Andong 36729, Republic of Korea;3. Insect Biotech Co. Ltd., Daejeon 34054, Republic of Korea;1. School of Environmental Studies, Jadavpur University, Kolkata 700 032, India;2. Department of Chemistry, Jadavpur University, Kolkata 700 032, India;3. Department of Chemical Engineering, National Institute of Technology, Mahatma Gandhi Road, Durgapur 713 209, India;4. Department of Chemical Engineering, McMaster University, 1280 Main St West, Hamilton, Ontario, L8S 4L8, Canada;1. Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran;2. Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran;3. Department of Microbiology, Faculty of Biological Science, Alzahra University, Tehran, Iran;1. Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India;2. Department of Botany, Pt. N.R.S. Govt College, Rohtak, 124001, Haryana, India;3. Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh, 123031, Haryana, India;1. School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso, Chile;2. Department of Biology, Faculty of Chemistry and Biology, Universidad de Santiago de Chile (USACH), Santiago, Chile;1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China;2. Department of Agricultural and Biosystems Engineering, Faculty of Agriculture, Benha University, P.O. Box 13736, Moshtohor, Qaluobia, Egypt;3. Faculty of Agriculture, University for Development Studies, Tamale, Ghana |
| |
| Abstract: | 2-Keto-d-gluconic acid (2-KGA) is a high-value-added product from lignocellulosic straw-based biorefining process obtained via cell catalysis using Gluconobacter oxydans (G. oxydans); nonetheless, the economical production has some limitations because of the formation of undesirable mixture products of keto-gluconic acid from glucose. In this study, it was demonstrated that pulse addition of hydrogen peroxide (H2O2) to synthetic media was beneficial to improve the conversion rate of glucose to 2-KGA by 1.47-fold in G. oxydans. Moreover, some lignocellulosic degradation compounds served as substitute and played the role of H2O2 on corn stover hydrolysate for the production of 2-KGA. By conducting qRT-PCR analysis, eight dehydrogenase genes were identified in G. oxydans, which were responsible for regulating 2-KGA production. Moreover, most of the identified genes were found to produce membrane-bound glucose dehydrogenase and gluconate 2-dehydrogenase, and were up-regulated by adding H2O2 or degradation compounds. These up-regulated genes could be responsible for directing cell catalysis of glucose to 2-KGA in G. oxydans. |
| |
| Keywords: | Cell catalysis Hydrogen peroxide Lignocellulosic hydrolysate |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
