Bioassay guided fractionation of bioactive metabolite from Corynascus verrucosus inhabiting Croton bonplandianus Baill

Mycoendophytes have been a promising source of bioactive metabolites for new developments in pharmaceuticals and industrial relevance. During the screening of mycoendophytes diversity from C. bonplandianus, bioactive C. verrucosus CBF 22 was isolated from the leaf. The bioactive isolate was subjected to fermentation and the ethyl acetate fraction was evaluated for antimicrobial activity by disc diffusion and microbroth dilution methods, followed by cytotoxicity using MTT assay. The results revealed significant activity against Gram negative bacteria, Gram positive bacteria, and human pathogenic dermatophytic fungi, C. albicans. The TLC Bioautography-Guided approach employed for the detection of antimicrobial metabolite from bioactive fraction was depicted by a zone of inhibition. Antimicrobial screening of mycoendophytes C. bonplandianus could envision a unique potential niche for bioactive compounds of pharmaceutical importance. These findings suggest C. verrucosus as a potent candidate for producing natural bioactives, to combat multidrug-resistant pathogens. This work is the first report on the incidence of C. verrucosus as mycoendophyte and its bioactive potential of inhabiting C. bonplandianus Baill.