Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826 |
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| Affiliation: | 1. Basque Institute for Agricultural Research and Development, NEIKER, Arkaute, Spain;2. TECNALIA, Technological Park of Alava, Leonardo Da Vinci 11, 01510, Miñano, Alava, Spain;1. Key Laboratory for Microorganisms and Biotransformation, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China;2. Angimmune LLC, Rockville, MD, USA;3. Zhoushan CSP Biosciences, Inc., Zhoushan, Zhejiang, China;1. Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan;2. Graduate School of Agriculture, Ibaraki University, Inashiki, Ibaraki, Japan;1. School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, 510006, People''s Republic of China;2. Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou, Guangdong, 510006, People''s Republic of China;3. AsiaPac (Dongguan) Bio-Technology Co., Ltd. No. 3, North Industrial Road, Songshan Lake National Hi-tech Industrial Development Zone, Dongguan City, Guangdong, 523808, People''s Republic of China;1. Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China;2. Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China;1. State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, People’s Republic of China;2. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, People’s Republic of China;3. Sports Health Center, Tongji University, Shanghai 200092, People’s Republic of China;4. Shanghai Collaborative Innovation Center for Biomanufacturing (SCICB), Shanghai 200237, People’s Republic of China;1. College of Biopharmaceutical and Food Engineering, Shangluo University, Shangluo, 726000, China;2. Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, 710119, China;3. College of Health Management, Shangluo University, Shangluo, 726000, China |
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| Abstract: | In this work a strategy for obtaining free amino-acids concentrate from an organic fraction of municipal solid waste compost and its use as a nitrogen source for lactic acid production, a compound widely used in different industries, using L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 strains is described. Enzymatic digestion is based on the combined action of endoprotease Alcalase 1.5 MG and exoprotease Flavourzyme 500 MG. The highest degree of hydrolysis obtained under the optimal conditions was 41%. The use of glucanase Viscozyme L prior to protein hydrolysis helped to reduce the viscosity of the solution and promote the action of proteases, increasing its hydrolysis degree by 76%. The hydrolysate contained all 21 amino-acids, making it ideal for lactic acid bacteria growth. During shake flask cultivations the culture media was complemented with glucose as carbon source. Finally, with the hydrolysate, a maximum lactic acid concentration of 9.0 ± 0.2 g·L−1 and 11.1 ± 0.1 g·L−1 for L. fermentum ATCC 9338 and L. plantarum NCIMB 8826 respectively was obtained after 27 h. The innovation of the approach lies in exploiting the overproduction of compost for the production of lactic acid. |
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| Keywords: | Organic fraction municipal solid waste Compost Enzymatic hydrolysis Protein hydrolysate Lactic acid |
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