Enhanced endoxylanase production by Myceliophthora thermophila using rice straw and its synergism with phytase in improving nutrition |
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| Institution: | 1. Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India;2. Department of Botany, Pt. N.R.S. Govt College, Rohtak, 124001, Haryana, India;3. Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh, 123031, Haryana, India;1. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan, 320, Taiwan;2. Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia;1. Fraunhofer Institute of Interfacial and Bioprocess Engineering, Stuttgart, Germany;2. Fraunhofer Center for Chemical-Biotechnological Processes, Leuna, Germany;1. Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran;2. Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran;3. Department of Microbiology, Faculty of Biological Science, Alzahra University, Tehran, Iran;1. Industrial Bio-materials Research Center, KRIBB, Daejeon 34141, Republic of Korea;2. Department of Biological Sciences, Andong National University, Andong 36729, Republic of Korea;3. Insect Biotech Co. Ltd., Daejeon 34054, Republic of Korea;1. School of Environmental Studies, Jadavpur University, Kolkata 700 032, India;2. Department of Chemistry, Jadavpur University, Kolkata 700 032, India;3. Department of Chemical Engineering, National Institute of Technology, Mahatma Gandhi Road, Durgapur 713 209, India;4. Department of Chemical Engineering, McMaster University, 1280 Main St West, Hamilton, Ontario, L8S 4L8, Canada;1. School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso, Chile;2. Department of Biology, Faculty of Chemistry and Biology, Universidad de Santiago de Chile (USACH), Santiago, Chile |
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| Abstract: | The goal of the present investigation was to attain the enhanced production of endoxylanase in submerged fermentation using different approaches followed by its utility in improving nutrition of wheat and rice flours along with phytase. Myceliophthora thermophila BJTLRMDU3 produced 51.70 U/mL of xylanase using rice straw as a substrate after optimization with ‘one variable at a time’ approach. After Plackett-Burman design study, sodium nitrate, K2HPO4 and Tween 20 were selected as critical factors and further optimized by response surface methodology. Increased xylanase production (80.15 U/mL) was attained with 2.5 % (w/v) sodium nitrate, 1.25 % (w/v) K2HPO4, and 2 % (v/v) Tween 20 at 40 °C. An overall 1.5-fold increase in xylanase production was achieved after statistical optimization. Applicability of M. thermophila xylanase (200 U/g flour) alone and in combination with phytase (15 U/g flour) from Aspergillus oryzae SBS50 in wheat and rice flours showed enhancement in nutritional qualities of both flours. About 45.67 %, 29.73 %, and 107.91 % increase in reducing sugars, soluble proteins and inorganic phosphate, respectively in wheat flour, while 94.16 %, 134.52 %, and 473.33 % increase in reducing sugars, soluble proteins and inorganic phosphate, respectively in rice flour was achieved at 60 °C and pH 5.0 by synergistic action of xylanase and phytase as compared to control having only xylanase. |
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| Keywords: | Xylanase Plackett-Burman Design Response surface methodology Phytase Wheat flour Rice flour |
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