Hydrogen sulfide-producing kinetics of Shewanella oneidensis in sulfite and thiosulfate respiration |
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| Affiliation: | 1. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India;2. Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India;3. National Centre for Cell Science, Pune, 411007, India;4. Centre for DNA Fingerprinting and Diagnostics, Hyderabad, 500 039, India;1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;2. Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China;3. Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China;4. Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China;1. College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China;2. Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;3. College of Civil Engineering, Lanzhou University of Technology, Lanzhou, 730050, China;4. Shenzhen DiDa Water Engineering Limited Company, Shenzhen, 518116, China;1. Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, China;2. Key Laboratory of Molecular Biophysics, Ministry of Education, 1037 Luoyu Road, Wuhan, 430074, China;3. Hubei Food and Medicine Resources Engineering Research Center, 1037 Luoyu Road, Wuhan, 430074, China |
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| Abstract: | Shewanella oneidensis is a model species for aquatic ecosystems and plays an important role in bioremediation, biofuel cell manufacturing and biogeochemical cycling. S. oneidensis MR-1 is able to generate hydrogen sulfide from various sulfur species; however, its catalytic kinetics have not been determined. In this study, five in-frame deletion mutants of S. oneidensis were constructed and their H2S-producing activities were analyzed. SirA and PsrA were the two major contributors to H2S generation under anoxic cultivation, and the optimum SO32− concentration for sulfite respiration was approximately 0.8 mM, while the optimum S2O32− concentration for thiosulfate respiration was approximately 0.4 mM. Sulfite and thiosulfate were observed to interfere with each other during respiration, and a high concentration of sulfite or thiosulfate chelated extracellular free-iron but did not repress the expression of sirA or psrA. Nitrite and nitrate were two preferred electron acceptors during anaerobic respiration; however, under energy-insufficient conditions, S. oneidensis could utilize multiple electron acceptors simultaneously. Elucidiating the stoichiometry of H2S production in S. oneidensis would be helpful for the application of this species in bioremediation and biofuel cell manufacturing, and would help to characterize the ecophysiology of sulfur cycling. |
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| Keywords: | Hydrogen sulfide Sulfite respiration Thiosulfate respiration SirA PsrA |
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