Biogenic synthesis of gold nanoparticles using Commiphora wightii and their cytotoxic effects on breast cancer cell line (MCF-7) |
| |
| Affiliation: | 1. Teresian Research Foundation, Teresian College, Siddarthanagar, Mysore - 570011, India;2. Department of Biochemistry, Indian Institute of Science, Bengaluru - 560012, India;3. Department of Biochemistry, Yuvarajas College, Mysore - 570 005, India;4. Institute of Research and Development, Duy Tan University, Da Nang 550000, Viet Nam;5. Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;1. Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea;2. Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea;1. Green Technology Group, Environmental Sciences Department, Faculty of Science, Alexandria University, 21511 Alexandria, Egypt;2. National Egyptian Biotechnology Experts Network, National Egyptian Academy for Scientific Research and Technology, Egypt;1. Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai, India;2. Biophysics Laboratory, Central Leather Research Institute, Adyar, Chennai, India;1. Bio-Products Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020 Tamil Nadu, India;2. Biomedical Instrumentation Science Loyola College, Chennai, 600034 Tamil Nadu, India;3. Department of Chemistry, Indian Institute of Technology/Madras, Chennai 600 036, India;4. Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 570-752 Republic of Korea |
| |
| Abstract: | The current study aimed at developing gold nanoparticles (AuNPs) using the aqueous extract of the medicinal plant Commiphora wightii. The phytosynthesized gold nanoparticles (Cw@AuNPs) were evaluated for their anticancer activity against MCF-7 breast cancer cell model. The formation of AuNPs by Commiphora wightii leaf extract was confirmed by UV–vis spectra where their surface plasmon resonance was found at 533 nm. Further characterization of Cw@AuNPs was done by transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, and fourier-transform infrared spectroscopy (FTIR) analysis. In vitro anticancer potential of thus obtained AuNPs was evaluated against MCF-7 and where the IC50 was found to be 66.11 μg/mL Further, apoptotic studies were carried out using ethidium bromide dual staining, DNA fragmentation, comet assay, and flow cytometry studies. Results revealed that Cw@AuNPs at higher concentration significantly increased the apoptotic cells when compared to control cells. Cell cycle analysis of MCF-7 cells confirmed the cell cycle arrest at G2/M phase. These results demonstrate that the biosynthesized Cw@AuNPs appear to be promising for therapeutical applications against breast cancer. |
| |
| Keywords: | Gold nanoparticles Cytotoxic Apoptosis DNA damage Phytosynthesis MCF-7 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
