Human gastric carcinoma cells targeting peptide-functionalized iron oxide nanoparticles delivery for magnetic resonance imaging |
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| Institution: | 1. Department of Radiology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China;2. Department of Stomatology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China;1. Department of Biochemistry and Clinical Laboratory, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;3. Student Research Committee and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;2. School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;1. Department of Mechanical and Electro-Mechanical Engineering, National I-Lan University, Yilan, Taiwan;2. Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia;3. Centre For Automotive Research and Tribology, Indian Institute of Technology, Delhi, India;4. Department of Mechanical Engineering, and Center for Engineering and Sustainable Development Research, De La Salle University, Philippines;5. School of Information, Systems and Modelling, Faculty of Engineering and Information Technology, University of Technology Sydney, NSW 2007, Australia;1. Department of Second Gynecology, Cangzhou Central Hospital, Cangzhou, Hebei Province, 061001, China;2. Department of Obstetrics, Dezhou Women and Children’s Hospital, No. 835 Dongdizhong Street, Dezhou City, Shandong, 253000, China;3. Department of Obstetrics, Taian City Central Hospital, Taian, Shandong Province, 271000, China;4. School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Malaysia;5. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar Perlis, Malaysia;6. Department of Obstetrics and Gynecology, Jinan Maternal & Child Care Health Hospital, No. 2, Jian Guo Xiao Jing 3 Road, Jinan, Shandong Province, 250001, China;1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China |
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| Abstract: | Iron oxide nanoparticles (IONPs) are broadly examined nanomaterials for their promising engagement of the progressive in biomedical application, for intense selective drug delivery and multimodal imaging. IONPs are commonly less price, and enhanced biocompatibility can be effectively functionalized with a broad range of functioning ligand, and have established to be active in improving clinical diagnostics tools and magnetic resonance imaging contrast agents. Consequently, IONPs could be used as a promising magnetic resonance imaging contrast. In this context, we have established an IONPs based framework for the multimodal in vitro imaging approach of gastric cancer cell lines that fast high level of glypican-3 protein (GLY-3) on the superficial. In this regards, a new GLY-3 peptide targeting model established and fabricated to IONPs. The aqueous property, biocompatibility profile and physical-chemical properties of the functionalized IONPs were characterised with various spectroscopical methods. The viability of the gastric SGC-7901 cells was examined by MTT assay. Further, the viability of the cells was evidenced through fluorescence staining methods. The binding ability and cellular uptake properties of naked IONPs and functionalized IONPs (GPC3@IONPs) were examined via laser scanning confocal microscopy (CLSM) in GLY-3 positive gastric cells (SGC-7901 cells). The obtained outcomes displayed that the GLY-3 functionalized IONPs remarkably improved the magnetic resonance imaging contrasts and were actively assured and occupied up by gastric cell lines without damaging the non-cancerous cells. |
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| Keywords: | Iron oxide nanoparticles Glypican-3 protein Gastric carcinoma Magnetic Resonance Imaging |
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