Atmospheric and room temperature plasma mutagenesis and astaxanthin production from sugarcane bagasse hydrolysate by Phaffia rhodozyma mutant Y1 |
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| Institution: | 1. Guangdong Provincial Engineering and Technology Research Center of Biopharmaceuticals, School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, China;2. College of Life and Geographic Sciences, Kashi University, Kashi 844000, China;3. Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, China;1. Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, China;2. Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China;3. Central Research Institute of Building and Construction, MCC Group, Co, Ltd;1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, PR China;2. Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, PR China;3. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China;4. The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China;5. SDIC Biotechnology Investment Co. Ltd, State Development and Investment Corporation, Beijing 100034, PR China;1. Faculty of Agro Based Industry, Universiti Malaysia Kelantan Jeli Campus, Jeli, Kelantan 17600, Malaysia;2. Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom;1. Department of Chemical Engineering, Faculty of Chemical Sciences, Autonomous University of Coahuila, Boulevard Venustiano Carranza SN, Saltillo, Coahuila, 25280, Mexico;2. Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece;3. Bioremediation Laboratory, Faculty of Biological Sciences, Autonomous University of Coahuila, Carretera Torreón-Matamoros, Torreón, Coahuila, 27000, Mexico;1. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India;2. Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India;1. College of Food Science and Engineering, Jilin Agricultural University, Changchun, China;2. Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China;3. National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China |
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| Abstract: | In this study, atmospheric and room temperature plasma and ultraviolet mutagenesis was studied for astaxanthin overproducing mutant. Phaffia rhodozyma mutant Y1 was obtained from the selection plate with 120 μmol/L diphenylamine as selection agent, and its carotenoid concentration and content were 54.38 mg/L and 5.38 mg/g, which were 19.02 % and 21.20 % higher than that of the original strain, respectively. Sugarcane bagasse hydrolysate was used for astaxanthin production by mutant Y1 at 22 °C and 220 rpm for 96 h, and the biomass and carotenoid concentration reached 12.65 g/L and 88.57 mg/L, respectively. Ultrasonication and cellulase were used to break cell wall and the parameters were optimized, achieving an astaxanthin extraction rate of 96.01 %. The present work provided a novel combined mutagenesis method for astaxanthin overproducing mutant and a green cell wall disruption process for astaxanthin extraction, which would play a solid foundation on the development of natural astaxanthin. |
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| Keywords: | Astaxanthin Atmospheric and room temperature plasma Sugarcane bagasse hydrolysate Cell wall disruption |
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