Egg White peptide KPHAEVVLR promotes skin fibroblasts migration and mice skin wound healing by stimulating cell membrane Hsp90α secretion |
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| Institution: | 1. School of Life Sciences, Jilin University, Changchun 130021, PR China;2. School of Stomatology, Jilin University, Changchun 130000, PR China;1. Key Laboratory of Textile Fibers and Products, Ministry of Education, P. R. C, College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, China;2. Third Institute of Oceanography, Ministry of Natural Resources, P. R. C, Xiamen 361005, China;3. High-Tech Organic Fibers Key Laboratory of Sichuan Province, Sichuan Textile Research Institute, Chengdu 610072, China;1. Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, PR China;2. Hefei Preschool Education College, Hefei, 230013, PR China;1. School of Life Sciences, Ludong University, Yantai 264025, China;2. School of Agriculture, Ludong University, Yantai 264025, China;1. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, 310015, PR China;2. Institute of Quality and Standard for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, PR China;3. School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China;1. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China;2. School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China;3. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China |
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| Abstract: | Egg white peptides are well known for their abundant species and biological properties. The aim of this study was to investigate the effect of egg white peptides on skin wound healing. We hydrolyzed the egg white powder using Alcalase and flavourzyme. Our transwell cell migration assay data showed that EWP2, which is one of the four fractions obtained from Sephadex G-15 isolation, promoted human skin fibroblast HSF cells migration. LC–MS/MS analysis results showed EWP2 were consist of 24 high abundant peptides. We randomly chose and synthesized four of these 24 peptides. Transwell assay data displayed that the egg white peptide KPHAEVVLR (KR-9) increased 8.2-fold of HSF cells migration at 200 μM compared with control. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sμlfophenyl)-2H-tetrazolium (MTS) assay results showed KR-9 did not lead to HSF cells over-proliferation. In vivo animal experiment data proved that KR-9 accelerated 50 % and 136 % mice skin wound healing rate at 50 μM and 100 μM. Furthermore, we explored the mechanism of KR-9. Our western blot and immunofluorescence results showed KR-9 enhanced the protein level of cell membrane heat shock protein 90α (Hsp90α). Thus, we conclude that egg white peptide KR-9 promotes skin fibroblasts migration and mice skin wound healing by stimulating cell membrane Hsp90α secretion. |
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| Keywords: | Egg white peptide Hydrolyzation Migration Wound healing Hsp90α |
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