哈茨木霉对水稻过氧化物酶及多酚氧化酶活性的影响*

哈茨木霉不仅对病原微生物有拮抗作用，而且能够诱导植物产生抗病性。采用酶动力学扫描及垂直板凝胶电泳技术，对水稻喷施木霉发酵液后，测定多酚氧化酶(PPO)、过氧化物酶(POD)活性和同工酶带图谱的动态变化。与对照组相比，哈茨木霉不仅能够提高PPO和POD酶的活力，而且还能诱导产生新的同工酶谱带，但不同浓度发酵液对诱导合成的酶作用不同。稀释50倍的发酵液对POD活力有较大的阳性效应。高浓度的发酵液对PPO活性有先抑制后出现提高的趋势，表明了该发酵液对PPO活性调节的程序性。

EFFECTS OF TRICHODERMA HARZIARUM ON POLYPHENOL OXIDASE AND PEROXIDASE IN RICE

Trichoderma harziaiarum plays a role of antagonic ability to pathogenic micr obes and of inducing plant to produce a resistivity of disease. In this paper, afte r the rice was sprinkled by zymolytic liquid of Trichoderma harziaiarum, the changes on the activities and isoenzymes of polyphenol oxidase (PPO) and pe roxidase (POD) were investigated. The results indicated that Trichoderma harz iarum could increase the activities of PPO and POD and produce new pattern of isoe nzymes; the different concentration of zymotic liquid had a different effects on the PPO and POD that were caused by the liquid; the 50 times diluted liquid had a greatly positive relation to the POD activity; and the higher concentration o f the liquid had a tendency that made the PPO activity inhibition and then incre ase, which denoted that the liquid took a scheduled adjustment play of the PPO a ctivity.